Inhibitory efficacy of bufadienolides on Na⁺,K⁺-pump activity versus cell proliferation.

ABSTRACT: Bufadienolides are cytotoxic drugs that may form the basis for anticancer agents. Due to structural and functional similarity to cardiotonic glycosides, application is restricted. We, therefore, investigated correlation of their putative anticancer effects with inhibition of Na⁺,K⁺pumps. The natural bufalin and three derivatives were tested. The anticancer effects of the drugs were checked by observing their inhibitory effects on proliferation of rat liver cancer cells using MTT assay. Inhibition of Na⁺,K⁺-pump was determined by measuring pump-mediated current of rat α1/β1 and α2/β1 Na⁺,K⁺pumps expressed in Xenopus oocytes. All tested bufadienolides inhibited cell proliferation and Na⁺,K⁺pump act

SUBMITTER: Xu Y 

PROVIDER: S-EPMC5600443 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature