Non-Native Metal Ion Reveals the Role of Electrostatics in Synaptotagmin 1-Membrane Interactions.

ABSTRACT: C2 domains are independently folded modules that often target their host proteins to anionic membranes in a Ca²⁺-dependent manner. In these cases, membrane association is triggered by Ca²⁺ binding to the negatively charged loop region of the C2 domain. Here, we used a non-native metal ion, Cd²⁺, in lieu of Ca²⁺ to gain insight into the contributions made by long-range Coulombic interactions and direct metal ion-lipid bridging to membrane binding. Using X-ray crystallography, NMR, Förster resonance energy transfer, and vesicle cosedimentation assays, we demonstrate that, although Cd²⁺ binds to the loop region of C2A/B domains of synaptotagmin 1 with high affinity, long-range Coulombic interactions are too weak to support membrane bindin

SUBMITTER: Katti S 

PROVIDER: S-EPMC5600830 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature