Nuclear CD24 Drives Tumor Growth and Is Predictive of Poor Patient Prognosis.

ABSTRACT: Elevated tumor expression of the cell surface GPI-linked CD24 protein signals poor patient prognosis in many tumor types. However, some cancer cells selected to be negative for surface CD24 (surCD24^-) still retain aggressive phenotypes in vitro and in vivo Here, we resolve this apparent paradox with the discovery of biologically active, nuclear CD24 (nucCD24) and finding that its levels are unchanged in surCD24^- cells. Using the complementary techniques of biochemical cellular fractionation and immunofluorescence, we demonstrate a signal for CD24 in the nucleus in cells from various histologic types of cancer. Nuclear-specific expression of CD24 (NLS-CD24) increased anchorage-independent growth in vitro and tumor formation in vivo Immunohis

SUBMITTER: Duex JE 

PROVIDER: S-EPMC5600841 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature