Mitochondrial Superoxide Increases Age-Associated Susceptibility of Human Dermal Fibroblasts to Radiation and Chemotherapy.

ABSTRACT: Elderly cancer patients treated with ionizing radiation (IR) or chemotherapy experience more frequent and greater normal tissue toxicity relative to younger patients. The current study demonstrates that exponentially growing fibroblasts from elderly (old) male donor subjects (70, 72, and 78 years) are significantly more sensitive to clonogenic killing mediated by platinum-based chemotherapy and IR (∼70%-80% killing) relative to young fibroblasts (5 months and 1 year; ∼10%-20% killing) and adult fibroblasts (20 years old; ∼10%-30% killing). Old fibroblasts also displayed significantly increased (2-4-fold) steady-state levels of O₂^•-, O₂ consumption, and mitochondrial membrane potential as well as significantly decreased (40%-50%) electron transport chain (ET

SUBMITTER: Mapuskar KA 

PROVIDER: S-EPMC5600863 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature