High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications.

ABSTRACT: The mechanical fingerprint of cells is inherently linked to the structure of the cytoskeleton and can serve as a label-free marker for cell homeostasis or pathologic states. How cytoskeletal composition affects the physical response of cells to external loads has been intensively studied with a spectrum of techniques, yet quantitative and statistically powerful investigations in the form of titration assays are hampered by the low throughput of most available methods. In this study, we employ real-time deformability cytometry (RT-DC), a novel microfluidic tool to examine the effects of biochemically modified F-actin and microtubule stability and nuclear chromatin structure on cell deformation in a human leukemia cell line (HL60). The high throughput of our method facilitates extensive titr

SUBMITTER: Golfier S 

PROVIDER: S-EPMC5601209 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature