Arp2/3 Complex Is Required for Macrophage Integrin Functions but Is Dispensable for FcR Phagocytosis and In Vivo Motility.

ABSTRACT: The Arp2/3 complex nucleates branched actin, forming networks involved in lamellipodial protrusion, phagocytosis, and cell adhesion. We derived primary bone marrow macrophages lacking Arp2/3 complex (Arpc2^-/-) and directly tested its role in macrophage functions. Despite protrusion and actin assembly defects, Arpc2^-/- macrophages competently phagocytose via FcR and chemotax toward CSF and CX3CL1. However, CR3 phagocytosis and fibronectin haptotaxis, both integrin-dependent processes, are disrupted. Integrin-responsive actin assembly and αM/β2 integrin localization are compromised in Arpc2^-/- cells. Using an in vivo system to observe endogenous monocytes migrating toward full-thickness ear wounds we found that Arpc2^-/- monocytes maintain cell spee

SUBMITTER: Rotty JD 

PROVIDER: S-EPMC5601320 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature