Indole Biodegradation in Acinetobacter sp. Strain O153: Genetic and Biochemical Characterization.

ABSTRACT: Indole is a molecule of considerable biochemical significance, acting as both an interspecies signal molecule and a building block of biological elements. Bacterial indole degradation has been demonstrated for a number of cases; however, very little is known about genes and proteins involved in this process. This study reports the cloning and initial functional characterization of genes (iif and ant cluster) responsible for indole biodegradation in Acinetobacter sp. strain O153. The catabolic cascade was reconstituted in vitro with recombinant proteins, and each protein was assigned an enzymatic function. Degradation starts with oxidation, mediated by the IifC and IifD flavin-dependent two-component oxygenase system. Formation of indigo is prevented by IifB, and

SUBMITTER: Sadauskas M 

PROVIDER: S-EPMC5601350 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature