Promoter DNA methylation analysis reveals a combined diagnosis of CpG-based biomarker for prostate cancer.

ABSTRACT:

Background

Prostate cancer (PCa) is the most common tumor in elderly men. However, the specificity and sensitivity of serum prostate-specific antigen levels in PCa diagnosis are controversial. This study aims to reveal a novel diagnosis biomarker in PCa.

Materials and methods

The differential methylated CpG sites between 423 primary PCa and 39 adjacent samples from The Cancer Genome Atlas (TCGA) on Illumina HumanMethylation 450 platform were analyzed. The diagnostic methylation markers were mined using the Prediction Analysis of Microarrays package in Bioconductor. Then, the Gene Expression Omnibus data was used for verification. Pyrosequencing was applied to improve methylation levels of five CpGs (cg06363129, cg08843517, cg05385513, cg07220448 and cg11417025).

Results</

SUBMITTER: Tang Y 

PROVIDER: S-EPMC5601644 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature