Rapid screening and identification of ACE inhibitors in snake venoms using at-line nanofractionation LC-MS.

ABSTRACT: This study presents an analytical method for the screening of snake venoms for inhibitors of the angiotensin-converting enzyme (ACE) and a strategy for their rapid identification. The method is based on an at-line nanofractionation approach, which combines liquid chromatography (LC), mass spectrometry (MS), and pharmacology in one platform. After initial LC separation of a crude venom, a post-column flow split is introduced enabling parallel MS identification and high-resolution fractionation onto 384-well plates. The plates are subsequently freeze-dried and used in a fluorescence-based ACE activity assay to determine the ability of the nanofractions to inhibit ACE activity. Once the bioactive wells are identified, the parallel MS data reveals the masses corresponding to the activities fou

SUBMITTER: Mladic M 

PROVIDER: S-EPMC5602078 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature