SHAPE analysis of the htrA RNA thermometer from Salmonella enterica.

ABSTRACT: RNA thermometers regulate expression of some genes involved in virulence of pathogenic bacteria such as Yersinia, Neisseria, and Salmonella They often function through temperature-dependent conformational changes that alter accessibility of the ribosome-binding site. The 5'-untranslated region (UTR) of the htrA mRNA from Salmonella enterica contains a very short RNA thermometer. We have systematically characterized the structure and dynamics of this thermometer at single-nucleotide resolution using SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension) assays. Our results confirm that the htrA thermometer adopts the predicted hairpin conformation at low temperatures, with conformational change occurring over a physiological temperatur

SUBMITTER: Choi EK 

PROVIDER: S-EPMC5602114 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature