MiR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells.

ABSTRACT:

Objective

This study aimed to explore the effect of miR-509-5p on pancreatic cancer progression and clarify the underlying mechanisms.

Methods

Real-time quantitative reverse transcription polymerase chain reaction was employed to determine miR-509-5p expression in pancreatic cancer tissues and noncancerous adjacent tissues. CCK-8 and Transwell experiments were employed to examine cellular proliferation, migration, and invasion after miR-509-5p mimic or inhibitor transfection. Bioinformatics tools were used to identify the target gene of miR-509-5p, and cotransfection of the target gene and miR-509-5p mimic was performed to determine the effect on the proliferation and migration of pancreatic cancer cells. A xenograft mouse model and histological analysis were also used to te

SUBMITTER: Li X 

PROVIDER: S-EPMC5602284 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature