CDK9 and SPT5 proteins are specifically required for expression of herpes simplex virus 1 replication-dependent late genes.

ABSTRACT: DNA replication greatly enhances expression of the herpes simplex virus 1 (HSV-1) γ2 late genes by still unknown mechanisms. Here, we demonstrate that 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB), an inhibitor of CDK9, suppresses expression of γ2 late genes with an IC₅₀ of 5 μm, which is at least 10 times lower than the IC₅₀ value required for inhibition of expression of early genes. The effect of DRB could not be explained by inhibition of DNA replication per se or loading of RNA polymerase II to late promoters and subsequent reduction of transcription. Instead, DRB reduces accumulation of γ2 late mRNA in the cytoplasm. In addition, we show that siRNA-mediated knockdown of the transcription factor SPT5, but not NELF-E, also gives rise to a specific inhi

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC5602406 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature