Autoinduction of protein expression.

ABSTRACT: This unit contains protocols for the use of lactose-derived autoinduction in Escherichia coli. The protocols allow for reproducible expression trials to be undertaken with minimal user intervention. A basic protocol covers production of unlabeled proteins for functional studies. Alternate protocols for selenomethionine labeling for X-ray structural studies, and multi-well plate growth for screening and optimization are also included.

SUBMITTER: Fox BG 

PROVIDER: S-EPMC5602607 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature