Porcine Esophageal Submucosal Gland Culture Model Shows Capacity for Proliferation and Differentiation.

ABSTRACT:

Background & aims

Although cells comprising esophageal submucosal glands (ESMGs) represent a potential progenitor cell niche, new models are needed to understand their capacity to proliferate and differentiate. By histologic appearance, ESMGs have been associated with both overlying normal squamous epithelium and columnar epithelium. Our aim was to assess ESMG proliferation and differentiation in a 3-dimensional culture model.

Methods

We evaluated proliferation in human ESMGs from normal and diseased tissue by proliferating cell nuclear antigen immunohistochemistry. Next, we compared 5-ethynyl-2'-deoxyuridine labeling in porcine ESMGs in vivo before and after esophageal injury with a novel in vitro porcine organoid ESMG model. Microarray analysis of ESMGs in culture was comp

SUBMITTER: von Furstenberg RJ 

PROVIDER: S-EPMC5602779 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature