Changes in DNA methylation in naive T helper cells regulate the pathophysiological state in minimal-change nephrotic syndrome.

ABSTRACT:

Background

DNA methylation plays a crucial role in regulating transcription, and changes in DNA methylation affect gene expression and disease development. Minimal change nephrotic syndrome (MCNS) has been reported to involve immunological disturbances. Since the characteristic features of the disease include recurrent relapse and sex and age preference, the disease pathogenesis may be partly related to epigenetic changes. However, little is known about these changes.

Methods

We analyzed genome-wide DNA methylation using the microarray-based integrated analysis of methylation by isoschizomers method. This method was used to evaluate methylation in monocytes (patient number; n = 6) and naïve T helper cells (n = 4) from the peripheral blood of MCNS patients both in relapse and

SUBMITTER: Kobayashi Y 

PROVIDER: S-EPMC5603023 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature