Changes in Corneal Innervation after HSV-1 Latency Established with Different Reactivation Phenotypes.

ABSTRACT:

Purpose of the study

We used a rabbit model infected with high phenotypic reactivators (HPRs) as well as recombinant HSV-1 (herpes simplex virus 1) with deletions to study their effect on corneal innervations after latency was established.

Materials and methods

Corneas from noninfected New Zealand white rabbits were used to obtain the entire map of corneal innervation. Others were inoculated with the HSV-1 strains McKrae, 17Syn+, or recombinant mutants with glycoprotein K (gK) deletion, or with infected early protein 0 (ICP0) deletion. The animals were euthanized at 124 to 125 days postinfection and the corneas were immunostained with a mouse monoclonal anti-βIII tubulin antibody. Images were acquired with a fluorescence microscope and corneal sub-basal nerve density was cal

SUBMITTER: He J 

PROVIDER: S-EPMC5603150 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature