Development of a UPLC-FLD Method for Detection of Aflatoxin B1 and M1 in Animal Tissue to Study the Effect of Curcumin on Mycotoxin Clearance Rates.

ABSTRACT: Aflatoxin B1 (AFB1) and its metabolite aflatoxin M1 (AFM1) are well-known carcinogens for humans and animals health. In this study, an ultra-high performance liquid chromatography linked with fluorescence detection (UPLC-FLD) method was optimized and validated. In addition, we investigated for the first time, the influence of curcumin on residue depletion of AFB1 and AFM1 in liver, kidney, and muscle tissues of broiler chickens and estimated a necessary clearance time required for AFB1 and AFM1 residues. The results showed that the average recoveries of AFB1 varied in liver, kidney, and muscles between 82.32-85.56, 85.34-88.45, and 84.88-89.73% respectively, while the average recoveries of AFM1 in liver, kidney, and muscles varied between 92.17-95.03, 94.12-97.21, and 95.32-98.51%, respect

SUBMITTER: Cui X 

PROVIDER: S-EPMC5603667 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature