A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.

ABSTRACT: RNA-RNA assembly governs key biological processes and is a powerful tool for engineering synthetic genetic circuits. Characterizing RNA assembly in living cells often involves monitoring fluorescent reporter proteins, which are at best indirect measures of underlying RNA-RNA hybridization events and are subject to additional temporal and load constraints associated with translation and activation of reporter proteins. In contrast, RNA aptamers that sequester small molecule dyes and activate their fluorescence are increasingly utilized in genetically encoded strategies to report on RNA-level events. Split-aptamer systems have been rationally designed to generate signal upon hybridization of two or more discrete RNA transcripts, but none directly function when expressed in vivo. We reasoned

SUBMITTER: Alam KK 

PROVIDER: S-EPMC5603824 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature