A-to-I RNA editing is developmentally regulated and generally adaptive for sexual reproduction in Neurospora crassa.

ABSTRACT: Although fungi lack adenosine deaminase acting on RNA (ADAR) enzymes, adenosine to inosine (A-to-I) RNA editing was reported recently in Fusarium graminearum during sexual reproduction. In this study, we profiled the A-to-I editing landscape and characterized its functional and adaptive properties in the model filamentous fungus Neurospora crassa A total of 40,677 A-to-I editing sites were identified, and approximately half of them displayed stage-specific editing or editing levels at different sexual stages. RNA-sequencing analysis with the Δstc-1 and Δsad-1 mutants confirmed A-to-I editing occurred before ascus development but became more prevalent during ascosporogenesis. Besides fungal-specific sequence and secondary structure preference, 63.5% of A-to-I edi

SUBMITTER: Liu H 

PROVIDER: S-EPMC5604002 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature