Quantitative tests of a reconstitution model for RNA folding thermodynamics and kinetics.

ABSTRACT: Decades of study of the architecture and function of structured RNAs have led to the perspective that RNA tertiary structure is modular, made of locally stable domains that retain their structure across RNAs. We formalize a hypothesis inspired by this modularity-that RNA folding thermodynamics and kinetics can be quantitatively predicted from separable energetic contributions of the individual components of a complex RNA. This reconstitution hypothesis considers RNA tertiary folding in terms of ΔG_align, the probability of aligning tertiary contact partners, and ΔG_tert, the favorable energetic contribution from the formation of tertiary contacts in an aligned state. This hypothesis predicts that changes in the alignment of tertiary contacts from different connecting he

SUBMITTER: Bisaria N 

PROVIDER: S-EPMC5604005 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature