Overexpression of DgWRKY4 Enhances Salt Tolerance in Chrysanthemum Seedlings.

ABSTRACT: High salinity seriously affects the production of chrysanthemum, so improving the salt tolerance of chrysanthemum becomes the focus and purpose of our research. The WRKY transcription factor (TF) family is highly associated with a number of processes of abiotic stress responses. We isolated DgWRKY4 from Dendranthema grandiflorum, and a protein encoded by this new gene contains two highly conserved WRKY domains and two C₂H₂ zinc-finger motifs. Then, we functionally characterized that DgWRKY4 was induced by salt, and DgWRKY4 overexpression in chrysanthemum resulted in increased tolerance to high salt stress compared to wild-type (WT). Under salt stress, the transgenic chrysanthemum accumulated less malondialdehyde, hydrogen peroxide (H_2<

SUBMITTER: Wang K 

PROVIDER: S-EPMC5604078 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature