Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells.

ABSTRACT: A promising nutraceutical, apigenin, was recently revealed to exhibit biological activity in inhibiting several types of cancer. The effects of apigenin on the growth inhibition and apoptosis of the cholangiocarcinoma HuCCA-1 cell line were investigated. Protein alterations subsequent to apigenin treatment were studied using a proteomic approach. The values of 20, 50 and 90% inhibition of cell growth (IC₂₀, IC₅₀ and IC₉₀) were determined by MTT cell viability assay. Apoptotic cell death was detected using two different methods, a flow cytometric analysis (Muse Cell Analyzer) and DNA fragmentation assay. A number of conditions including attached and detached cells were selected to perform two-dimensional gel electrophoresis (2-DE) to study the alterations in

SUBMITTER: Subhasitanont P 

PROVIDER: S-EPMC5604180 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature