Programmable Microfluidic Synthesis of Over One Thousand Uniquely Identifiable Spectral Codes.

ABSTRACT: Encoded microparticles have become a powerful tool for a wide array of applications, including high-throughput sample tracking and massively parallel biological multiplexing. Spectral encoding, where particles are encoded with distinct luminescence spectra, provides a particularly appealing encoding strategy because of the ease of reading codes and assay flexibility. To date, spectral encoding has been limited in the number of codes that can be accurately resolved. Here, we demonstrate an automated 5-dimensional spectral encoding scheme using lanthanide nanophosphors that is capable of producing isotropic spherical microparticles with up to 1,100 unique codes, which we term MRBLEs (Microspheres with Ratiometric Barcode Lanthanide Encoding). We further develop a quantitative framework for e

SUBMITTER: Nguyen HQ 

PROVIDER: S-EPMC5604317 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature