Molecular characterization, expression analysis and heterologous expression of two translationally controlled tumor protein genes from Cucumis sativus.

ABSTRACT: The translationally controlled tumor protein (TCTP) is a family of abundant and ubiquitous proteins involved in several important primary functions. Cucumbers harbor two TCTP genes, CsTCTP1 and CsTCTP2; however, their functional roles remain unclear. In this study, we isolated CsTCTP1 and CsTCTP2 (XP-004134215 and XP-004135602, respectively) promoters, full-length cDNA and genomic sequences from Cucumis sativus. Bioinformatics analysis, containing cis-acting elements, structural domains, phylogenetic tree and conserved motifs, suggested the conservation and divergence of CsTCTP1 and CsTCTP2, thus providing knowledge regarding their functions. Expression analysis indicated that CsTCTP1 and CsTCTP2 exhibited tissue-specific expression and were regulated by biotic or abiotic stresses in C. sa

SUBMITTER: Meng XN 

PROVIDER: S-EPMC5605047 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature