Reversion of High-level Mecillinam Resistance to Susceptibility in Escherichia coli During Growth in Urine.

ABSTRACT: Mecillinam (amdinocillin) is a β-lactam antibiotic used to treat uncomplicated urinary tract infections (UTIs). We have previously shown that inactivation of the Escherichia coli cysB gene is the major cause of mecillinam resistance (Mec^R) in clinical isolates. In this study, we used different E. coli strains (laboratory and clinical isolates) that were Mec^R due to cysB mutations to determine how mecillinam susceptibility was affected during growth in urine compared to growth in the commonly used growth medium Mueller Hinton (MHB). We also examined mecillinam susceptibility when bacteria were grown in urine obtained from 48 different healthy volunteers. Metabolome analysis was done on the urine samples and the association between the mecillinam susceptibility patterns

SUBMITTER: Thulin E 

PROVIDER: S-EPMC5605379 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature