Engraftment and Repopulation Potential of Late Gestation Fetal Rat Hepatocytes.

ABSTRACT:

Background

The limited availability of donor organs has led to a search for alternatives to liver transplantation to restore liver function and bridge patients to transplantation. We have shown that the proliferation of late gestation (embryonic day 19) fetal rat hepatocytes is mitogen-independent and that mechanisms regulating mRNA translation, cell cycle progression, and gene expression differ from those of adult rat hepatocytes. In the present study, we investigated whether E19 fetal hepatocytes can engraft and repopulate an injured adult liver.

Methods

Fetal hepatocytes were isolated using a monoclonal antibody against a hepatic surface protein, leucine amino peptidase (LAP). LAP+ and LAP- fractions were analyzed by immunofluorescence and microarray. Immunopurified E19 l

SUBMITTER: Boylan JM 

PROVIDER: S-EPMC5605407 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature