Transcriptome Analysis of Mycobacteria-Specific CD4⁺ T Cells Identified by Activation-Induced Expression of CD154.

ABSTRACT: Analysis of Ag-specific CD4⁺ T cells in mycobacterial infections at the transcriptome level is informative but technically challenging. Although several methods exist for identifying Ag-specific T cells, including intracellular cytokine staining, cell surface cytokine-capture assays, and staining with peptide:MHC class II multimers, all of these have significant technical constraints that limit their usefulness. Measurement of activation-induced expression of CD154 has been reported to detect live Ag-specific CD4⁺ T cells, but this approach remains underexplored and, to our knowledge, has not previously been applied in mycobacteria-infected animals. In this article, we show that CD154 expression identifies adoptively transferred or endogenous Ag-specific CD4⁺

SUBMITTER: Kunnath-Velayudhan S 

PROVIDER: S-EPMC5605459 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature