Granule cells control recovery from classical conditioned fear responses in the zebrafish cerebellum.

ABSTRACT: Although previous studies show that the cerebellum is involved in classical fear conditioning, it is not clear which components in the cerebellum control it or how. We addressed this issue using a delayed fear-conditioning paradigm with late-stage zebrafish larvae, with the light extinguishment as the conditioned stimulus (CS) and an electric shock as the unconditioned stimulus (US). The US induced bradycardia in the restrained larvae. After paired-associate conditioning with the CS and US, a substantial population of the larvae displayed CS-evoked bradycardia responses. To investigate the roles of the zebrafish cerebellum in classical fear conditioning, we expressed botulinum toxin or the Ca²⁺ indicator GCaMP7a in cerebellar neurons. The botulinum-toxin-dependent inhibition of

SUBMITTER: Matsuda K 

PROVIDER: S-EPMC5605521 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature