Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models.

ABSTRACT: The strong societal urge to reduce the use of experimental animals, and the biological differences between rodent and human skin, have led to the development of alternative models for healthy and diseased human skin. However, the limited availability of primary keratinocytes to generate such models hampers large-scale implementation of skin models in biomedical, toxicological, and pharmaceutical research. Immortalized cell lines may overcome these issues, however, few immortalized human keratinocyte cell lines are available and most do not form a fully stratified epithelium. In this study we compared two immortalized keratinocyte cell lines (N/TERT1, N/TERT2G) to human primary keratinocytes based on epidermal differentiation, response to inflammatory mediators, and the development of norma

SUBMITTER: Smits JPH 

PROVIDER: S-EPMC5605545 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature