Phosphorylation of Suppressor of Hairless impedes its DNA-binding activity.

ABSTRACT: Notch signalling activity governs cellular differentiation in higher metazoa, where Notch signals are transduced by the transcription factor CSL, called Suppressor of Hairless [Su(H)] in Drosophila. Su(H) operates as molecular switch on Notch target genes: within activator complexes, including intracellular Notch, or within repressor complexes, including the antagonist Hairless. Mass spectrometry identified phosphorylation on Serine 269 in Su(H), potentially serving as a point of cross-regulation by other signalling pathways. To address the biological significance, we generated phospho-deficient [Su(H)^S269A] and phospho-mimetic [Su(H)^S269D] variants: the latter displayed reduced transcriptional activity despite unaltered protein interactions with co-activators and -re

SUBMITTER: Nagel AC 

PROVIDER: S-EPMC5605572 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature