Agdc1p - a Gallic Acid Decarboxylase Involved in the Degradation of Tannic Acid in the Yeast Blastobotrys (Arxula) adeninivorans.

ABSTRACT: Tannins and hydroxylated aromatic acids, such as gallic acid (3,4,5-trihydroxybenzoic acid), are plant secondary metabolites which protect plants against herbivores and plant-associated microorganisms. Some microbes, such as the yeast Arxula adeninivorans are resistant to these antimicrobial substances and are able to use tannins and gallic acid as carbon sources. In this study, the Arxula gallic acid decarboxylase (Agdc1p) which degrades gallic acid to pyrogallol was characterized and its function in tannin catabolism analyzed. The enzyme has a higher affinity for gallic acid (K_m -0.7 ± 0.2 mM, k_cat -42.0 ± 8.2 s^-1) than to protocatechuic acid (3,4-dihydroxybenzoic acid) (K_m -3.2 ± 0.2 mM, k_cat -44.0 ± 3.2 s^-1

SUBMITTER: Meier AK 

PROVIDER: S-EPMC5605622 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature