Two NAD-linked redox shuttles maintain the peroxisomal redox balance in Saccharomyces cerevisiae.

ABSTRACT: In Saccharomyces cerevisiae, peroxisomes are the sole site of fatty acid β-oxidation. During this process, NAD⁺ is reduced to NADH. When cells are grown on oleate medium, peroxisomal NADH is reoxidised to NAD⁺ by malate dehydrogenase (Mdh3p) and reduction equivalents are transferred to the cytosol by the malate/oxaloacetate shuttle. The ultimate step in lysine biosynthesis, the NAD⁺-dependent dehydrogenation of saccharopine to lysine, is another NAD⁺-dependent reaction performed inside peroxisomes. We have found that in glucose grown cells, both the malate/oxaloacetate shuttle and a glycerol-3-phosphate dehydrogenase 1(Gpd1p)-dependent shuttle are able to maintain the intraperoxisomal redox balance. Single mutants in MDH3 or GPD1 grow on lysine-d

SUBMITTER: Al-Saryi NA 

PROVIDER: S-EPMC5605654 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature