Detection of clinically relevant copy number alterations in oral cancer progression using multiplexed droplet digital PCR.

ABSTRACT: Copy number alterations (CNAs), a common genomic event during carcinogenesis, are known to affect a large fraction of the genome. Common recurrent gains or losses of specific chromosomal regions occur at frequencies that they may be considered distinctive features of tumoral cells. Here we introduce a novel multiplexed droplet digital PCR (ddPCR) assay capable of detecting recurrent CNAs that drive tumorigenesis of oral squamous cell carcinoma. Applied to DNA extracted from oral cell lines and clinical samples of various disease stages, we found good agreement between CNAs detected by our ddPCR assay with those previously reported using comparative genomic hybridization or single nucleotide polymorphism arrays. Furthermore, we demonstrate that the ability to target specific locations of th

SUBMITTER: Hughesman CB 

PROVIDER: S-EPMC5605662 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature