Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis.

ABSTRACT:

Background

Spirocerca lupi, the dog oesophageal nematode, causes a potentially fatal disease in domestic dogs, and is currently clinically diagnosed by coproscopy and oesophagoscopy. To date, a single molecular method, a semi-nested PCR, targeting the cox1 gene, has been developed to aid in the diagnosis of spirocercosis. The present study describes three novel high-resolution melt (HRM) quantitative PCR (qPCR) assays targeting fragments of the ITS1, 18S and cytb loci of S. lupi. The performance of these molecular assays in feces was compared to fecal flotation and to the previously described cox1 gene semi-nested PCR in 18 fecal samples from dogs with clinical oesophageal spirocercosis diagnosed by oesophagoscopy.

Results

The HRM qPCR for ITS1 and 18S were both able to dete

SUBMITTER: Rojas A 

PROVIDER: S-EPMC5606040 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature