Primary cilia modulate TLR4-mediated inflammatory responses in hippocampal neurons.

ABSTRACT:

Background

The primary cilium is an organelle that can act as a master regulator of cellular signaling. Despite the presence of primary cilia in hippocampal neurons, their function is not fully understood. Recent studies have demonstrated that the primary cilium influences interleukin (IL)-1β-induced NF-κB signaling, ultimately mediating the inflammatory response. We, therefore, investigated ciliary function and NF-κB signaling in lipopolysaccharide (LPS)-induced neuroinflammation in conjunction with ciliary length analysis.

Methods

Since TLR4/NF-κB signaling is a well-known inflammatory pathway, we measured ciliary length and inflammatory mediators in wild type (WT) and TLR4^-/- mice injected with LPS. Next, to exclude the effects of microglial TLR4, we examined t

SUBMITTER: Baek H 

PROVIDER: S-EPMC5606072 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature