Proteinuria without albuminuria: urinary protein excretion by a subset of patients with burn injuries.

ABSTRACT:

Background

There is disagreement regarding the utility of urinary albumin excretion as a marker for capillary injury in patients with severe burn injuries. We examined protein components in urine specimens from patients with burn injury.

Methods

Detailed analysis was performed for a set of 5 urine specimens selected based on a high ratio of albumin-sized molecules by size-exclusion HPLC (Accumin) versus albumin by immunoassay methods. Specimens were analyzed for total protein, alpha(1)-microglobulin, alpha(1)-acid glycoprotein, cystatin C, and retinol-binding protein. Urine components were analyzed by chromatographic and electrophoretic methods. Major components were identified by mass spectrometry of tryptic peptides.

Results

A subset of urine specimens had increased

SUBMITTER: Sviridov D 

PROVIDER: S-EPMC5606198 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature