Subcellular Imaging of Voltage and Calcium Signals Reveals Neural Processing In Vivo.

ABSTRACT: A mechanistic understanding of neural computation requires determining how information is processed as it passes through neurons and across synapses. However, it has been challenging to measure membrane potential changes in axons and dendrites in vivo. We use in vivo, two-photon imaging of novel genetically encoded voltage indicators, as well as calcium imaging, to measure sensory stimulus-evoked signals in the Drosophila visual system with subcellular resolution. Across synapses, we find major transformations in the kinetics, amplitude, and sign of voltage responses to light. We also describe distinct relationships between voltage and calcium signals in different neuronal compartments, a substrate for local computation. Finally, we demonstrate that ON and OFF selectivity, a key feature of

SUBMITTER: Yang HH 

PROVIDER: S-EPMC5606228 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature