Functional analyses of putative PalS (Palindromic Self-recognition) motifs in bacterial Hfq.

ABSTRACT: The bacterial protein Hfq has been linked to nucleic acid metabolism and signaling, however its explicit role has been elusive. Recently it was proposed that the C-termini of Hfq subunits in Hfq₆ complexes could be involved in functional interactions with other Hfq hexamers and/or nucleic acids. To test the proposed model of the native Hfq complex experimentally, we genetically engineered chimeric Hfq₆ complexes, in which C-termini of bacterial Hfq subunits were substituted with a sequence derived from human histone H2B (hH2B) that includes multiple functionally significant amino acids whose modifications have been linked to carcinogenesis. We demonstrate that this substitution results in an enhanced formation of dodecameric assemblies by the Hfq-hH2B hybrid - a resul

SUBMITTER: Jackson TC 

PROVIDER: S-EPMC5606250 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature