Automatic and adaptive heterogeneous refractive index compensation for light-sheet microscopy.

ABSTRACT: Optical tissue clearing has revolutionized researchers' ability to perform fluorescent measurements of molecules, cells, and structures within intact tissue. One common complication to all optically cleared tissue is a spatially heterogeneous refractive index, leading to light scattering and first-order defocus. We designed C-DSLM (cleared tissue digital scanned light-sheet microscopy) as a low-cost method intended to automatically generate in-focus images of cleared tissue. We demonstrate the flexibility and power of C-DSLM by quantifying fluorescent features in tissue from multiple animal models using refractive index matched and mismatched microscope objectives. This includes a unique measurement of myelin tracks within intact tissue using an endogenous fluorescent reporter where typica

SUBMITTER: Ryan DP 

PROVIDER: S-EPMC5606987 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature