Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial activity.

ABSTRACT: In the present study, role of various physicochemical parameters influencing the production of antimicrobial pigment prodigiosin from Serratia nematodiphila RL2 was determined and optimized. The pigment-producing strain was isolated and based on molecular characterization (16S rRNA sequencing), was identified as S. nematodiphila RL2. The pigment produced by S. nematodiphila RL2 was characterized by thin layer chromatography (Rf 0.94), spectrophotometrically (λ_max 535 nm) and identified as prodigiosin. Optimization of production parameters of prodigiosin revealed, nutrient broth medium supplemented with lactose and yeast extract at 1% concentration each, have a positive effect on the bacterial growth (10.25-4.6 mg/ml DCW) as well as pigment production

SUBMITTER: Gondil VS 

PROVIDER: S-EPMC5607078 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature