In vivo imaging system for explants analysis-A new approach for assessment of cell transplantation effects in large animal models.

ABSTRACT:

Introduction

Despite spectacular progress in cellular transplantology, there are still many concerns about the fate of transplanted cells. More preclinical studies are needed, especially on large animal models, to bridge the translational gap between basic research and the clinic. Herein, we propose a novel approach in analysis of cell transplantation effects in large animals explants using in vivo imaging system (IVIS®) or similar equipment.

Material and methods

In the in vitro experiment cells labeled with fluorescent membrane dyes: DID (far red) or PKH26 (orange) were visualized with IVIS®. The correlation between the fluorescence signal and cell number with or without addition of minced muscle tissue was calculated. In the ex vivo study urethras obtained from goats after

SUBMITTER: Zarychta-Wisniewska W 

PROVIDER: S-EPMC5607129 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature