Chimeric 14-3-3 proteins for unraveling interactions with intrinsically disordered partners.

ABSTRACT: In eukaryotes, several "hub" proteins integrate signals from different interacting partners that bind through intrinsically disordered regions. The 14-3-3 protein hub, which plays wide-ranging roles in cellular processes, has been linked to numerous human disorders and is a promising target for therapeutic intervention. Partner proteins usually bind via insertion of a phosphopeptide into an amphipathic groove of 14-3-3. Structural plasticity in the groove generates promiscuity allowing accommodation of hundreds of different partners. So far, accurate structural information has been derived for only a few 14-3-3 complexes with phosphopeptide-containing proteins and a variety of complexes with short synthetic peptides. To further advance structural studies, here we propose a novel approach b

SUBMITTER: Sluchanko NN 

PROVIDER: S-EPMC5607241 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature