Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates.

ABSTRACT: Antibodies are indispensable tools for basic research as well as diagnostic and therapeutic applications. Consequently, the development of alternative manufacturing strategies which circumvent the hurdles connected to conventional antibody production technologies is of enormous interest. To address this issue, we demonstrate the synthesis of complex antibody formats, in particular immunoglobulin G (IgG) and single-chain variable fragment Fc fusion (scFv-Fc), in a microsome-containing cell-free system based on translationally active chinese hamster ovary (CHO) cell lysates. To mimic the environment for antibody folding and assembly present in living cells, antibody genes were fused to an endoplasmic reticulum (ER)-specific signal sequence. Signal-peptide induced translocation of antibody po

SUBMITTER: Stech M 

PROVIDER: S-EPMC5607253 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature