Identification and characterization of a novel Sso7d scaffold-based binder against Notch1.

ABSTRACT: Notch signaling has important functions in regulating cell growth and development, misregulation of which has been implicated in various cancers. Monoclonal antibodies (mAbs) targeting Notch protein activity have already moved into clinical trials. However due to the limitations associated with cost and productivity of mAbs, there has been a surge in the development of complementary approaches that are based on non-antibody scaffolds. Non-antibody scaffolds are small proteins that are stable and can be engineered to develop high-affinity binders against specific targets of interest. Here we describe the isolation and characterization of a novel Notch1-binding protein, N9, obtained by screening of a combinatorial library based on the ultra-stable Sso7d scaffold. N9 targets the extracellular

SUBMITTER: Gocha T 

PROVIDER: S-EPMC5607287 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature