Molecular characterization of the duck enteritis virus US10 protein.

ABSTRACT:

Background

There is little information regarding the duck enteritis virus (DEV) US10 gene and its molecular characterization.

Methods

Duck enteritis virus US10 was amplified and cloned into the recombinant vector pET32a(+). The recombinant US10 protein was expressed in Escherichia coli BL21 cells and used to immunize rabbits for the preparation of polyclonal antibodies. The harvested rabbit antiserum against DEV US10 was detected and analyzed by agar immunodiffusion. Using this antibody, western blotting and indirect immunofluorescence analysis were used to analyze the expression level and subcellular localization of US10 in infected cells at different time points. Quantitative reverse-transcription PCR (qRT-PCR) and pharmacological inhibition tests were used to ascertain th

SUBMITTER: Zhang D 

PROVIDER: S-EPMC5607491 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature