Topological organization and dynamic regulation of human tRNA genes during macrophage differentiation.

ABSTRACT:

Background

The human genome is hierarchically organized into local and long-range structures that help shape cell-type-specific transcription patterns. Transfer RNA (tRNA) genes (tDNAs), which are transcribed by RNA polymerase III (RNAPIII) and encode RNA molecules responsible for translation, are dispersed throughout the genome and, in many cases, linearly organized into genomic clusters with other tDNAs. Whether the location and three-dimensional organization of tDNAs contribute to the activity of these genes has remained difficult to address, due in part to unique challenges related to tRNA sequencing. We therefore devised integrated tDNA expression profiling, a method that combines RNAPIII mapping with biotin-capture of nascent tRNAs. We apply this method to the study of dynami

SUBMITTER: Van Bortle K 

PROVIDER: S-EPMC5607496 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature