Pseudomonas stutzeri as an alternative host for membrane proteins.

ABSTRACT:

Background

Studies on membrane proteins are often hampered by insufficient yields of the protein of interest. Several prokaryotic hosts have been tested for their applicability as production platform but still Escherichia coli by far is the one most commonly used. Nevertheless, it has been demonstrated that in some cases hosts other than E. coli are more appropriate for certain target proteins.

Results

Here we have developed an expression system for the heterologous production of membrane proteins using a single plasmid-based approach. The gammaproteobacterium Pseudomonas stutzeri was employed as a new production host. We investigated several basic microbiological features crucial for its handling in the laboratory. The organism belonging to bio-safety level one is a close r

SUBMITTER: Sommer M 

PROVIDER: S-EPMC5607611 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature