Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children.

ABSTRACT:

Background

Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS.

Patients and methods

This case-control study was performed on 138 patients with NS and 150 healthy children. Genomic DNA was extracted from whole blood using salting out method. Polymorphism of the ET-1 rs5370 G>T (lys198Asn) polymorphism detected by T-ARMS-PCR as well as PCR-RFLP method.

Results

The results showed that the genotype and allelic frequencies of the ET-1 rs5370 G>T variant were not significantly different between cases and controls. Furthermore, subgroup analysis showed that rs5370 G>T variant was not associat

SUBMITTER: Hashemi M 

PROVIDER: S-EPMC5607974 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature