Intercalated disc in failing hearts from patients with dilated cardiomyopathy: Its role in the depressed left ventricular function.

ABSTRACT: Alterations in myocardial structure and reduced cardiomyocyte adhesions have been previously described in dilated cardiomyopathy (DCM). We studied the transcriptome of cell adhesion molecules in these patients and their relationships with left ventricular (LV) function decay. We also visualized the intercalated disc (ID) structure and organization. The transcriptomic profile of 23 explanted LV samples was analyzed using RNA-sequencing (13 DCM, 10 control [CNT]), focusing on cell adhesion genes. Electron microscopy analysis to visualize ID structural differences and immunohistochemistry experiments of ID proteins was also performed. RT-qPCR and western blot experiments were carried out on ID components. We found 29 differentially expressed genes, most of all, constituents of the ID structur

SUBMITTER: Ortega A 

PROVIDER: S-EPMC5608295 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature