Immunopathology of childhood celiac disease-Key role of intestinal epithelial cells.

ABSTRACT:

Background & aims

Celiac disease is a chronic inflammatory disease of the small intestine mucosa due to permanent intolerance to dietary gluten. The aim was to elucidate the role of small intestinal epithelial cells in the immunopathology of celiac disease in particular the influence of celiac disease-associated bacteria.

Methods

Duodenal biopsies were collected from children with active celiac disease, treated celiac disease, and clinical controls. Intestinal epithelial cells were purified and analyzed for gene expression changes at the mRNA and protein levels. Two in vitro models for human intestinal epithelium, small intestinal enteroids and polarized tight monolayers, were utilized to assess how interferon-γ, interleukin-17A, celiac disease-associated bacteria and gluten

SUBMITTER: Pietz G 

PROVIDER: S-EPMC5608296 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature